ZymoBIOMICS Gut Microbiome Standard Purification Detection of multiple targets in

Detection of multiple targets in a single reaction (multiplex RT-qPCR) is feasible using TaqMan probes (not provided)

Staphylococcus aureus - 14%

library preps

it is difficult to achieve both species-level resolution and broad phylogenetic coverage

dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini

ZymoBIOMICS Gut Microbiome Standard Purification Detection of multiple targets inThe ZymoBIOMICS Gut Microbiome Standard is an accurately quantified microbial community mimicking the human gut microbiome. The challenges it presents to microbiomics profiling allows researchers to validate protocols and fine tune them for gut microbiome research and act as a positive control for sample processing.