Zymoprep Yeast Plasmid Miniprep II His-tagged Protein Purification The purified RNA is eluted
Description
The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations
without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps
Optimal Zymolyase activity is at 30° - 37°C
The outgrowth step in yeast transformation protocols is often critical for increasing overall yeast transformation efficiencies
Binding conditions are adjusted using this buffer to selectively recover ≥ 300 bp
Zymoprep Yeast Plasmid Miniprep II His-tagged Protein Purification The purified RNA is elutedThe Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The
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