CpG Methylase (M. Sssl) Name:CpG Methylase (M. Sssl) Desulphonation and clean-up of the

Desulphonation and clean-up of the converted DNA is performed on a spin-column

Intended for use with conventional style 1

is induced during growth

and concentration of 10 µg per prep of single stranded DNA and/or RNA (e

and proteinases that are often used for ChIP

CpG Methylase (M. Sssl) Name:CpG Methylase (M. Sssl) Desulphonation and clean-up of theCpG Methylase completely methylates all cytosines in double stranded, non methylated, and hemimethylated DNA in a 5' CpG 3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis.